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qPCR validation of ECM-related gene expression. (A–J) The mRNA expression levels of A2M, LAMA1, VIT, COL15A1, TNS1, TGM2, CTSB, ITGB6, TIMP1, and VCAN were quantified in samples from the Normal, PBS, and hAEC-EVs groups using qRT-PCR. Statistical analysis was performed using one-way ANOVA. Data are presented as the means ± SEM (n = 3). *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Targeted proteomic and bioinformatic investigation of extracellular matrix remodeling in hAEC-EV-mediated corneal repair

doi: 10.3389/fbioe.2026.1772203

Figure Lengend Snippet: qPCR validation of ECM-related gene expression. (A–J) The mRNA expression levels of A2M, LAMA1, VIT, COL15A1, TNS1, TGM2, CTSB, ITGB6, TIMP1, and VCAN were quantified in samples from the Normal, PBS, and hAEC-EVs groups using qRT-PCR. Statistical analysis was performed using one-way ANOVA. Data are presented as the means ± SEM (n = 3). *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001.

Article Snippet: TIMP1 , 16644-1-AP , Proteintech , 1:1,000.

Techniques: Biomarker Discovery, Gene Expression, Expressing, Quantitative RT-PCR

Western blot validation of ECM-related gene expression at the protein level. (A–J) The protein expression levels of A2M, LAMA1, VIT, COL15A1, TNS1, TGM2, CTSB, TIMP1, and VCAN were analyzed in samples from the Normal, PBS, and hAEC-EVs groups by Western blot. Protein band intensities were quantified and normalized to GAPDH. Statistical analysis was performed using one-way ANOVA. Data are presented as the means ± SEM (n = 3). *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Targeted proteomic and bioinformatic investigation of extracellular matrix remodeling in hAEC-EV-mediated corneal repair

doi: 10.3389/fbioe.2026.1772203

Figure Lengend Snippet: Western blot validation of ECM-related gene expression at the protein level. (A–J) The protein expression levels of A2M, LAMA1, VIT, COL15A1, TNS1, TGM2, CTSB, TIMP1, and VCAN were analyzed in samples from the Normal, PBS, and hAEC-EVs groups by Western blot. Protein band intensities were quantified and normalized to GAPDH. Statistical analysis was performed using one-way ANOVA. Data are presented as the means ± SEM (n = 3). *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001.

Article Snippet: TIMP1 , 16644-1-AP , Proteintech , 1:1,000.

Techniques: Western Blot, Biomarker Discovery, Gene Expression, Expressing